Gene Expression / Transcriptome
Gene Expression / Transcriptome
Digital Gene Expression, Transcriptome
Skuldtech provides construction of gene expression libraries by using high throughput sequencing technology and proprietary Bioinformatics tools.
By using our company’s platform, researchers can obtain a complete view of the transcriptional activity, coding or non-coding RNA, in eukaryotic or prokaryotic cells without any prior knowledge on genomes and establish profiles for accurately identifying and quantifying each expressed gene.
Skuldtech supports a broad and complementary portfolio of gene expression analysis products which include:
- quantitative coding RNA,
- noncoding RNA expression and small RNA species from “Digital Gene Expression” data (SAGE-like method),
- assessing exon-level expression from RNA-seq data, and lastly,
- paired-end reads which are used for solving repeats by jumping across them and clarifying the order of unique flanking regions or revealing trans-splicing processes.
Some gene annotations of non-model organisms need to be consolidated for the gene expression analysis. In this case, Skuldtech provides construction of EST libraries based on the 454 Life Sciences/Roche Diagnostics platform which provides more than 400,000 sequences of 400 nucleotide lengths.
Skuldtech’s high throughput data acquisition platform is associated with powerful Bioinformatics programs, which have been developed for processing and analyzing these specific types of genomic data. They can easily be integrated into any other Bioinformatics database or any gene analysis Bioinformatics tool.
Skuldtech’s Bioinformatics programs are accessed through an active and user-friendly website and can be used for processing and validating experimental data, identifying expressed genes, comparing gene profiles and for giving a biological sense to observed variations through the integration of data in their biological context (interaction and biological networks, inter-species comparative analysis, etc.).
Regarding its gene profile analysis programs, Skuldtech has processed and analyzed DGE sequencing data from several international academic laboratories, as well as for human, animal or plant organisms.
Please contact our commercial department if you have any questions or requests or if you wish to receive a quotation.
Quantitative RT PCR
Skuldtech provides real-time PCR expertise and services including:
- qPCR performance: From samples to the result analysis.
- qPCR optimization: Analysis and optimization of your PCR protocols regarding targeted DNA sequences.
- qPCR result analysis: Analysis and interpretation of qPCR results.
- Integrated work: All the above steps can be combined as a single service for processing samples and providing results.
Please contact our commercial department if you have any questions or requests or if you wish to receive a quotation.
ESTs, ChIP and small RNA
Skuldtech provides:
- Expressed Sequence Tags (EST) libraries: Detailed libraries of all expressed genes are constructed by sequencing 400,000 sequences (length of ~ 400bp) for exploring gene diversity, SNPs, etc.
- ChIP and Small RNA services.
Gene expression data analysis and implementation
Skuldtech’s platform enables to analyze gene expression profiles and accurately identify and quantify each expressed gene. This high throughput data acquisition platform is associated with powerful Bioinformatics programs, which have been developed to process and analyze these specific kinds of genomic data and which can be easily integrated with any other Bioinformatics database or any gene analysis Bioinformatics tool.
Skuldtech’s Bioinformatics programs are accessed through an active and user-friendly website and can be used for processing and validating experimental data, identifying expressed genes, comparing gene profiles and for giving a biological sense to observed variations through the integration of data in their biological context (interaction and biological networks, inter-species comparative analysis, etc.).
Analyzed gene profiles can also be integrated into a Non-Array gene profile database developed by Skuldtech, containing more than 1,000 gene profiles at present.
Thanks to these gene profile analysis programs, Skuldtech has processed and analyzed many DGE sequencing data from several international research laboratories, as well as for human, animal or plant organisms.
Please contact our commercial department if you have any questions or requests or if you wish to receive a quotation.
Gene expression profile database
Thanks to its Bioinformatics platform, Skuldtech provides access to a large gene profile database containing more than 1,000 gene expression patterns.
Biotag, Skuldtech’s active and user-friendly website access tool, is being used for developing this database so that end-users can access relevant biological data.
Therefore, each user can easily search and compare his selected gene profiles and transcriptome libraries, design biomarker sets in a specific tissue and/or tumour, and anticipate drug effects on complete organisms, etc.
Biotag allows each user to:
- Associate a gene, a mRNA or a simple acid nucleic sequence, with a well-defined tag signature, and to measure the relevance of the information obtained (sense or antisense mRNA, pseudogene, repeated sequences, etc.),
- Visualize the expression level of any tag signature in any organisms: Homo sapiens, Mus musculus, etc.
- Visualize each tag signature based on tissue, pathology or functional criteria.
- Establish an achieve genomic homology between Homo sapiens and Mus musculus organisms.
- Associate a gene with its potential antisense strand.
- Search for potential limited punctual genetic polymorphisms.
- Identify potential splicing gene events.
- etc.
Click here to see the distribution of these gene profiles per organism.
The list of gene profiles is available on www.skuldtech.com/GEOST.php
To discover a demo of Biotag, please click on: Demo
Please contact our commercial department if you have any questions or requests or if you wish to receive a quotation.
Customized genomic database
On the basis of its gene expression profile database and Biotag, an active and user-friendly website access tool, Skuldtech provides customized websites dedicated to researchers.
For any gene from his/her selection, a user can obtain a form including several genomic data – he/she can discover the different profiles, classified by tissue or pathology, in which this gene is expressed.
In addition, the user can easily make detailed comparisons between two genes (or more) from his/her selection and assess their potential co-expression in the pathology/tissue under study.
To discover a demo version of this customized web tool, please click on: Demo
Please contact our commercial department if you have any questions or requests or if you wish to receive a quotation.
[Re]Sequencing: Genome, ORFome, Splicing, SNPs…
Skuldtech provides the following services:
- High Throughput sequencing: Genome Sequencer FLX (Roche Diagnostics / 454 Life Sciences) or 1G Sequencer (Illumina).
- DNA sequence analysis: DNA sequencing for clinical trials, genomics, pharmacogenomics and diagnostic studies, etc.
- DNA genotyping: DNA genotyping for population verification and population assessment in clinical trials and research, etc.
- Specimen Identification: Using SNP and short tandem repeat (STR) genotyping methods to positively identify study specimens, etc.
- Large sequencing: Full genome sequencing service including organism sequencing: Virus Bacteria, Parasites etc.
- Customized sequencing for short fragments.
- Sequencing Engineering: Sample processing issue following-up project results expectation. Development of customized analysis.
- Micro-satellite sequencing: micro-satellite sequencing and analysis using the Ssr Analysis Tool.
- Sequence identification: Have you isolated an unknown sequence, or a sequence which has mutated or which has not been properly identified or annotated? Skuldtech provides you with all the accurate information required for pursuing your diagnosis. From De Novo DNA fragments to new sequenced DNA.
- Mutation investigation: Do you have a clinical case which does not correspond to a standard and well-established genetic diagnosis? Skuldtech provides investigation of the question by sequencing new or unknown mutations.
Skuldtech provides drug repurposing services in association with Aureus Pharma.
Please contact our commercial department for any question, demand or quotation.
Genotyping: Microsatellites analysis
Based on its 10 years of experience in genomics, Skuldtech proposes microsatellite DNA loci or tandem repeats – VNTRs (Variable Number Tandem Repeats) and genotyping analysis services.
Skuldtech’s scope of services in microsatellite DNA loci development includes:
- Designing and developing a microsatellite DNA loci bank specifically dedicated to the species under study.
- Sequencing microsatellite DNA loci for population genotyping purposes.
- Optimizing and customizing protocols for the analysis of microsatellite DNA loci from specific species.
- Primer optimization.
Within the last 5 years, Skuldtech has genotyped several species through microsatellite DNA loci analysis.
Please contact our commercial department if you have any questions or requests or if you wish to receive a quotation.
PCR
Skuldtech’s scope of services includes:
- Primer design: Designing primers for PCR and real-time PCR on the basis of the sequences to be amplified.
- Synthesis of oligonucleotides: Synthesis of primers or any other oligonucleotides upon request.
- Amplification of DNA: Amplification through PCR techniques.
- Electrophoresis revelation: Revelation by using electrophoresis gels.
- Revelation: For an accurate PCR results analysis.
- PCR optimization: Analysis and optimization of your PCR protocols regarding targeted DNA sequences.
- Integrated work: All the above steps can be added and combined as a single service for processing samples and providing results.
Please contact our commercial department if you have any questions or requests or if you wish to receive a quotation.
DNA EXTRACTION AND QUANTIFICATION
Skuldtech is specialized in:
- DNA extraction: DNA extraction from any samples associated with clinical trials or research.
- DNA quantification: DNA quantification associated with clinical trials or research.
Please contact our commercial department if you have any questions or requests or if you wish to receive a quotation.
AMPLIFICATION – DETECTION
Skuldtech is specialized in:
- The construction of cDNA libraries: Reverse Transcription of mRNA to cDNA.
